Bioisis

"Folded Dimer of PF1911"

Experimental SAS Curve

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Low_res_thumbnail

Experimental Mass

67,200 Da

Experimental Details for BID:  1FERHP
Experiment ID: 1
Collected at: ALS BL 12.3.1
Contributors: Rambo, R.P. ,  Hura, G.L. ,  Menon, A.L. ,  Hammel, M. ,  Adams, M.W.W. ,  Tainer, J.A.
Solution structure of a Ferredoxin NADP reductase from Pyrococcus furiosus.

Electron Pair Distribution

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       Dmax → 101 Å


Guinier Plot

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     Guinier Rg → 30.9 Å

Real Space Rg → 31.2 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


DAMMIN/F Model

DAMMIN/F result was determined with the following:

Space GroupP2
NSD | RMSD0.535 
variance(NSD | RMSD)0.1 
Number of Models in Average10 

GASBOR Model

GASBOR result was determined with the following:

Space GroupP2
Chi1.22
NSD | RMSD1.19 
variance(NSD | RMSD)0.1 
Number of Models in Average10 
Superimposed model10 

Additional Experimental Details
Title

Folded Dimer of PF1911

Description

Solution structure of a Ferredoxin NADP reductase from Pyrococcus furiosus.

Publication

Rapid and robust proteomics-scale solution structural analyses determined efficiently by X-ray scattering (SAXS), Nature Methods, submitted.

Contributors

Rambo, R.P. ,  Hura, G.L. ,  Menon, A.L. ,  Hammel, M. ,  Adams, M.W.W. ,  Tainer, J.A.

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: PF1911

Annotation: putative hydrogenase, recombinant with N-terminal His-tag

AHHHHHHGSM YKILEKKEIA MRNTWYKVYA PHVAKKVQPG QFVIVRAFPN GERIPLTPVM WDREEGWIVL IVFTRGKTTM RMAVELKEGD SLLNVAGPLG TPVPMEKFGK ILAIGAYTGI VEVYPIAKAW QEIGNDVTTL HVTFEPMVIL KEELEKAVTR HIVEPVPLNP NQDFLANMKN VSQRLKEKVR ELLESEDWDL VFMVGPVGDQ KQVFEVVKEY GVPMKVDLHP IMVDGTGMCG ACRVTVGGEV KFACVDGPEF DAYQVDWDEL IHRVGFYAKL EKLALEKYME ELKAKGVI
categoryamino acid composition(%)
HydrophobicI(5.4) V(12.4) L(7.7) M(4.4) A(6.4) G(7.7) P(5.7)
AromaticF(3.7) W(2.0) Y(3.0)
HydrophilicR(3.7) K(8.4) E(9.4) D(4.4) Q(2.7) N(2.7) H(3.7) S(1.3) T(4.4) C(1.0)