Bioisis

"E.c. MnmE/MnmG complex in the nucleotide free state"

Experimental SAS Curve

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Experimental Mass

207,000 Da

Experimental Details for BID:  MnmEGP
Experiment ID: 106
Collected at: SWING, Soleil, Paris
Contributors: Fislage, M ,  Brosens, E ,  Garcia-pino, A ,  Versees, W
E.c MnmE and E.c. MnmG were mixed in equimolar amounts and purified via gelfiltration. Sample homogeneity was assesed using SEC-MALS.
8 mg/ml protein solution injected on a KW404 gel filtration column prior to data collection

Electron Pair Distribution

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       Dmax → 184 Å


Guinier Plot

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     Guinier Rg → 52.3 Å

Real Space Rg → 55.6 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 3.1.


Additional Experimental Details
Title

E.c. MnmE/MnmG complex in the nucleotide free state

Description

E.c MnmE and E.c. MnmG were mixed in equimolar amounts and purified via gelfiltration. Sample homogeneity was assesed using SEC-MALS.

Publication

SAXS analysis of the tRNA-modifying enzyme complex MnmE/MnmG reveals a novel interaction mode and GTP-induced oligomerization, Fislage M, et al., Nucleic Acids Res. 2014;42(9):5978-92. doi: 10.1093/nar/gku213. Epub 2014 Mar 14.,PMID: 24634441

Contributors

Fislage, M ,  Brosens, E ,  Garcia-pino, A ,  Versees, W

Genomics and Proteomics

The experiment is composed of 2 genes/ORFs

Abbreviated name: EcMnmE

Annotation: E.. coli MnmE, GTPase involved in tRNA modification

MSDNDTIVAQ ATPPGRGGVG ILRISGFKAR EVAETVLGKL PKPRYADYLP FKDADGSVLD QGIALWFPGP NSFTGEDVLE LQGHGGPVIL DLLLKRILTI PGLRIARPGE FSERAFLNDK LDLAQAEAIA DLIDASSEQA ARSALNSLQG AFSARVNHLV EALTHLRIYV EAAIDFPDEE IDFLSDGKIE AQLNDVIADL DAVRAEARQG SLLREGMKVV IAGRPNAGKS SLLNALAGRE AAIVTDIAGT TRDVLREHIH IDGMPLHIID TAGLREASDE VERIGIERAW QEIEQADRVL FMVDGTTTDA VDPAEIWPEF IARLPAKLPI TVVRNKADIT GETLGMSEVN GHALIRLSAR TGEGVDVLRN HLKQSMGFDT NMEGGFLARR RHLQALEQAA EHLQQGKAQL LGAWAGELLA EELRLAQQNL SEITGEFTSD DLLGRIFSSF CIGK
categoryamino acid composition(%)
HydrophobicI(7.3) V(5.5) L(12.1) M(1.5) A(11.9) G(9.0) P(3.7)
AromaticF(3.5) W(0.9) Y(0.7)
HydrophilicR(7.0) K(3.1) E(7.9) D(7.3) Q(4.0) N(2.9) H(2.2) S(4.8) T(4.4) C(0.2)

Abbreviated name: EcMnmG

Annotation: tRNA uridine 5-carboxymethylaminomethyl modification enzyme

MFYPDPFDVI IIGGGHAGTE AAMAAARMGQ QTLLLTHNID TLGQMSCNPA IGGIGKGHLV KEVDALGGLM AKAIDQAGIQ FRILNASKGP AVRATRAQAD RVLYRQAVRT ALENQPNLMI FQQAVEDLIV ENDRVVGAVT QMGLKFRAKA VVLTVGTFLD GKIHIGLDNY SGGRAGDPPS IPLSRRLREL PLRVGRLKTG TPPRIDARTI DFSVLAQQHG DNPMPVFSFM GNASQHPQQV PCYITHTNEK THDVIRSNLD RSPMYAGVIE GVGPRYCPSI EDKVMRFADR NQHQIFLEPE GLTSNEIYPN GISTSLPFDV QMQIVRSMQG MENAKIVRPG YAIEYDFFDP RDLKPTLESK FIQGLFFAGQ INGTTGYEEA AAQGLLAGLN AARLSADKEG WAPARSQAYL GVLVDDLCTL GTKEPYRMFT SRAEYRLMLR EDNADLRLTE IGRELGLVDD ERWARFNEKL ENIERERQRL KSTWVTPSAE AAAEVNAHLT APLSREASGE DLLRRPEMTY EKLTTLTPFA PALTDEQAAE QVEIQVKYEG YIARQQDEIE KQLRNENTLL PATLDYRQVS GLSNEVIAKL NDHKPASIGQ ASRISGVTPA AISILLVWLK KQGMLRRSA
categoryamino acid composition(%)
HydrophobicI(6.0) V(5.7) L(9.9) M(2.9) A(10.0) G(7.9) P(5.4)
AromaticF(3.2) W(0.6) Y(2.7)
HydrophilicR(7.5) K(3.8) E(6.7) D(5.4) Q(5.6) N(4.0) H(1.7) S(4.8) T(5.6) C(0.6)