Bioisis

"A. aeolicus MnmG"

Experimental SAS Curve

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Experimental Mass

168,000 Da

Experimental Details for BID:  MnmG1P
Experiment ID: 109
Collected at: SWING, Soleil, Paris
Contributors: Fislage, M ,  Brosens, E ,  Garcia-pino, A ,  Versees, W
SAXS data of A. aeolicus MnmG. MnmG was expressed in E. coli and purified via Ni-NTA affinity chromatography followed by gel filtration. Sample homogeneity was assesed using SEC-MALS.
8 mg/ml protein solution injected on a KW404 gel filtration column prior to data collection

Electron Pair Distribution

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       Dmax → 139 Å


Guinier Plot

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     Guinier Rg → 42.3 Å

Real Space Rg → 43.0 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 1.8.


Additional Experimental Details
Title

A. aeolicus MnmG

Description

SAXS data of A. aeolicus MnmG. MnmG was expressed in E. coli and purified via Ni-NTA affinity chromatography followed by gel filtration. Sample homogeneity was assesed using SEC-MALS.

Publication

SAXS analysis of the tRNA-modifying enzyme complex MnmE/MnmG reveals a novel interaction mode and GTP-induced oligomerization, Fislage M, et al., Nucleic Acids Res. 2014;42(9):5978-92. doi: 10.1093/nar/gku213. Epub 2014 Mar 14.,PMID: 24634441

Contributors

Fislage, M ,  Brosens, E ,  Garcia-pino, A ,  Versees, W

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: AaMnmG

Annotation: tRNA uridine 5-carboxymethylaminomethyl modification enzyme

MAWVVDEFDV VVIGGGHAGI EAALAAARMG AKTAMFVLNA DTIGQMSCNP AIGGIAKGIV VREIDALGGE MGKAIDQTGI QFKMLNTRKG KAVQSPRAQA DKKRYREYMK KVCENQENLY IKQEEVVDII VKNNQVVGVR TNLGVEYKTK AVVVTTGTFL NGVIYIGDKM IPGGRLGEPR SEGLSDFYRR FDFPLIRFKT GTPARLDKRT IDFSALEVAP GDDPPPKFSF WTEPVGSYWF PKGKEQVNCW ITYTTPKTHE IIRKNLHRTA LYGGLIKGIG PRYCPSIEDK IVKFPDKERH QIFLEPEGLD TIEIYPNGLS TSLPEEVQWE MYRSIPGLEN VVLIRPAYAI EYDVVPPTEL YPTLETKKIR GLFHAGNFNG TTGYEEAAGQ GIVAGINAAL RAFGKEPIYL RRDESYIGVM IDDLTTKGVT EPYRLFTSRS EYRLYIRQDN AILRLAKLGR ELGLLSEEQY KLVKELEREI EKWKEFYKSE RVSVAVGGDT RSYSVATLMT MNYTLDDVKE KFGYEVPQHP YVKEEVEIQL KYEPYIERER KLNEKLKKLE DTKIPPDIDY DKIPGLTKEA REKLKKFKPI TVGQASRIDG ITPAAITALL VYLGKLD
categoryamino acid composition(%)
HydrophobicI(7.8) V(7.1) L(7.9) M(1.9) A(6.5) G(8.6) P(5.5)
AromaticF(3.4) W(1.0) Y(5.0)
HydrophilicR(6.0) K(8.4) E(8.9) D(5.0) Q(2.8) N(3.1) H(1.0) S(3.2) T(6.2) C(0.6)