Bioisis

"Folded Dimer of PF0699"

Experimental SAS Curve

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Experimental Mass

32,000 Da

Experimental Details for BID:  1KINHP
Experiment ID: 2
Collected at: ALS BL 12.3.1
Contributors: Rambo, R.P. ,  Hura, G.L. ,  Menon, A.L. ,  Adams, M.W.W. ,  Tainer, J.A.
Part of a set of samples prepared recombinantly. Has his-tag attached(see sequence). Scattering profiles show similarity to a shikamate kinase from E. coli. Data is not on an absolute scale and is a compilation of several data sets collected at different energies.

Electron Pair Distribution

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       Dmax → 74 Å


Guinier Plot

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     Guinier Rg → 23.7 Å

Real Space Rg → 23.3 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


DAMMIN/F Model

DAMMIN/F result was determined with the following:

Space GroupP1
NSD | RMSD0.479 
variance(NSD | RMSD)0.1 
Number of Models in Average10 

GASBOR Model

GASBOR result was determined with the following:

Space GroupP2
Chi1.74
NSD | RMSD0.89 
variance(NSD | RMSD)0.1 
Number of Models in Average10 
Superimposed model10 

Additional Experimental Details
Title

Folded Dimer of PF0699

Description

Part of a set of samples prepared recombinantly. Has his-tag attached(see sequence). Scattering profiles show similarity to a shikamate kinase from E. coli. Data is not on an absolute scale and is a compilation of several data sets collected at different energies.

Publication

Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS). Nat Methods. 2009 Aug;6(8):606-12.

Contributors

Rambo, R.P. ,  Hura, G.L. ,  Menon, A.L. ,  Adams, M.W.W. ,  Tainer, J.A.

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: PF0699

Annotation: hypothetical protein

AHHHHHHGSS SLFKSSWNRF ILLFFSQISW NQVVFSSPNL LNNLSIKNTP TPPMKTAKPA ESRTSLVPTR AIKPPIGLPI DTPTMLNTCY RPTEVPLTSL GTSVTSRVLA SIRPESAIPK APNVPSCTPG VLTTIPSKKA EKDIIDE
categoryamino acid composition(%)
HydrophobicI(7.5) V(5.4) L(8.8) M(1.4) A(5.4) G(2.7) P(12.2)
AromaticF(3.4) W(1.4) Y(0.7)
HydrophilicR(4.1) K(6.1) E(3.4) D(2.0) Q(1.4) N(5.4) H(4.1) S(12.9) T(10.2) C(1.4)