Bioisis

"PF1733 - Aggregated"

Experimental SAS Curve

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Experimental Mass

100,000,000 Da

Experimental Details for BID:  6HYPHP
Experiment ID: 26
Collected at: ALS BL 12.3.1 SIBYLS
Contributors: Hura, G.L. ,  Adams, M.W.W. ,  Mennon, A.L.
The scattering profile has no features. The Dmax is challenging to define. The Kratky plot is hopeful but this is most likely a mixture with some species of large size.

Electron Pair Distribution

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       Dmax → 280 Å


Guinier Plot

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     Guinier Rg → 58.2 Å

Real Space Rg → 63.0 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The scattering profile has no features. The Dmax is challenging to define. The Kratky plot is hopeful but this is most likely a mixture with some species of large size.

The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


No Model

No structural model was used to interpret the SAXS data, please read the following:

The scattering profile has no features. The Dmax is challenging to define. The Kratky plot is hopeful but this is most likely a mixture with some species of large size.


Additional Experimental Details
Title

PF1733 - Aggregated

Description

The scattering profile has no features. The Dmax is challenging to define. The Kratky plot is hopeful but this is most likely a mixture with some species of large size.

Publication

Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS). Nat Methods. 2009 Aug;6(8):606-12.

Contributors

Hura, G.L. ,  Adams, M.W.W. ,  Mennon, A.L.

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: PF1733

Annotation: hypothetical protein

AHHHHHHGSE KKFIAIYDLE SDKIEIIDDK FRFKCIEDCG KCCIYNEIPL REEDIQKILS LGYEEEYFVD YTKMFYRGPK FLGYGMKKRP FDDACVFLDP ETKKCRIYEH RPVACRIYPF VLVKHEKKLE IYVKEDPRCP GINSPDGEGI VEIIKKYFIP IIEELKGEIN GNRRSRQEDF ISFNRRLETV V
categoryamino acid composition(%)
HydrophobicI(11.5) V(4.7) L(5.2) M(1.0) A(2.1) G(5.8) P(5.2)
AromaticF(6.3) W(0.0) Y(5.8)
HydrophilicR(6.8) K(10.5) E(11.5) D(6.8) Q(1.0) N(2.6) H(4.2) S(3.1) T(1.6) C(4.2)