Bioisis

"PF1372 - Pyrococcus furiosus "

Experimental SAS Curve

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Low_res_thumbnail

Experimental Mass

39,476 Da

Experimental Details for BID:  3HYPHP
Experiment ID: 30
Collected at: ALS bl12.3.1 SIBYLS
Contributors: Hura, G.L. ,  Rambo, R.P. ,  Hammel, M. ,  Menon, A.L. ,  Rambo, R.P. ,  Admas, M.W.W.
PF1372 has no sequence homology to a protein of similar size with available atomic resolution structure. It is tetrameric and the SAXS shape has been determined using 4-fold symmetry.

Electron Pair Distribution

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       Dmax → 75 Å


Guinier Plot

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     Guinier Rg → 23.5 Å

Real Space Rg → 23.16 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


DAMMIN/F Model

DAMMIN/F result was determined with the following:

Space GroupP1
NSD | RMSD0.464 
variance(NSD | RMSD)0.1 
Number of Models in Average

GASBOR Model

GASBOR result was determined with the following:

Space GroupP4
Chi1.67
NSD | RMSD0.87 
variance(NSD | RMSD)0.1 
Number of Models in Average
Superimposed model

Additional Experimental Details
Title

PF1372 - Pyrococcus furiosus

Description

PF1372 has no sequence homology to a protein of similar size with available atomic resolution structure. It is tetrameric and the SAXS shape has been determined using 4-fold symmetry.

Publication

Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS). Nat Methods. 2009 Aug;6(8):606-12.

Contributors

Hura, G.L. ,  Rambo, R.P. ,  Hammel, M. ,  Menon, A.L. ,  Rambo, R.P. ,  Admas, M.W.W.

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: PF1372

Annotation: hypothetical protein

AHHHHHHGST EIRYYKIGED RFKISEDEVA RRELRVAKVS DDVIQIQEEV HGIIALVGAT SSVNIKKEEL KELVKLVREE FGWDI
categoryamino acid composition(%)
HydrophobicI(10.6) V(10.6) L(5.9) M(0.0) A(5.9) G(5.9) P(0.0)
AromaticF(2.4) W(1.2) Y(2.4)
HydrophilicR(7.1) K(8.2) E(14.1) D(5.9) Q(2.4) N(1.2) H(8.2) S(5.9) T(2.4) C(0.0)