Bioisis

"PF1061 Pyrococcus furiosus monomeric product"

Experimental SAS Curve

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Experimental Mass

8,592 Da

Experimental Details for BID:  2HYPHP
Experiment ID: 31
Collected at: ALS bl12.3.1 SIBYLS
Contributors: Rambo, R.P. ,  Hura, G.L. ,  Menon, A.L. ,  Adams, M.W.W. ,  Tainer, J.A.
C-alpha model NMR strucutre 1RWS.

Electron Pair Distribution

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       Dmax → 60 Å


Guinier Plot

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     Guinier Rg → 17.1 Å

Real Space Rg → 16.34 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 2.3.


DAMMIN/F Model

DAMMIN/F result was determined with the following:

Space GroupP1
NSD | RMSD0.538 
variance(NSD | RMSD)0.2 
Number of Models in Average

GASBOR Model

GASBOR result was determined with the following:

Space GroupP1
Chi1.38
NSD | RMSD0.76 
variance(NSD | RMSD)0.2 
Number of Models in Average
Superimposed model

Additional Experimental Details
Title

PF1061 Pyrococcus furiosus monomeric product

Description

C-alpha model NMR strucutre 1RWS.

Publication

in press Nature Methods Rapid and robust proteomics-scale solution structural analyses determined efficiently by x-ray scattering (SAXS)

Contributors

Rambo, R.P. ,  Hura, G.L. ,  Menon, A.L. ,  Adams, M.W.W. ,  Tainer, J.A.

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: PF1061

Annotation: hypothetical protein

AHHHHHHGSK MIKVKVIGRN IEKEIEWREG MKVRDILRAV GFNTESAIAK VNGKVVLEDD EVKDGDFVEV IPVVSGG
categoryamino acid composition(%)
HydrophobicI(9.1) V(15.6) L(2.6) M(2.6) A(5.2) G(10.4) P(1.3)
AromaticF(2.6) W(1.3) Y(0.0)
HydrophilicR(5.2) K(10.4) E(10.4) D(6.5) Q(0.0) N(3.9) H(7.8) S(3.9) T(1.3) C(0.0)