Bioisis

"PF1291 - Pyrococcus furiosus "

Experimental SAS Curve

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Low_res_thumbnail

Experimental Mass

117,521 Da

Experimental Details for BID:  2HYPOP
Experiment ID: 34
Collected at: ALS bl12.3.1 SIBYLS
Contributors: Hura, G.L. ,  Rambo, R.P. ,  Hammel, M. ,  Menon, A.L. ,  Adams, M.W.W. ,  Tainer, J.A.
PF1291 forms a tetramer in solution. The tetrameric form has no atomic resolution structure with sequence homology. Its shape appears to be from a novel fold.

Electron Pair Distribution

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       Dmax → 110 Å


Guinier Plot

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     Guinier Rg → 35.6 Å

Real Space Rg → 35.37 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


DAMMIN/F Model

DAMMIN/F result was determined with the following:

Space GroupP1
NSD | RMSD0.672 
variance(NSD | RMSD) 
Number of Models in Average

GASBOR Model

GASBOR result was determined with the following:

Space GroupP4
Chi1.22
NSD | RMSD1.08 
variance(NSD | RMSD) 
Number of Models in Average
Superimposed model

Additional Experimental Details
Title

PF1291 - Pyrococcus furiosus

Description

PF1291 forms a tetramer in solution. The tetrameric form has no atomic resolution structure with sequence homology. Its shape appears to be from a novel fold.

Publication

in press Nature Methods Rapid and robust proteomics-scale solution structural analyses determined efficiently by x-ray scattering (SAXS)

Contributors

Hura, G.L. ,  Rambo, R.P. ,  Hammel, M. ,  Menon, A.L. ,  Adams, M.W.W. ,  Tainer, J.A.

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: No Locus Name Available

Annotation: hypothetical protein

AHHHHHHGSV YVAVLANIAG NLPALTAALS RIEEMREEGY EIEKYYILGN IVGLFPYPKE VIEVIKDLTK KENVKIIRGK YDQIIAMSDP HATDPGYIDK LELPGHVKKA LKFTWEKLGH EGREYLRDLP IYLVDKIGGN EVFGVYGSPI NPFDGEVLAE QPTSYYEAIM RPVKDYEMLI VASPMYPVDA MTRYGRVVCP GSVGFPPGKE HKATFALVDV DTLKPKFIEV EYDKKIIEER IRAEGLPEEI IKILYHGGRP
categoryamino acid composition(%)
HydrophobicI(9.2) V(8.1) L(7.7) M(2.3) A(6.5) G(8.5) P(7.3)
AromaticF(2.7) W(0.4) Y(6.5)
HydrophilicR(4.2) K(8.1) E(10.0) D(5.0) Q(0.8) N(2.3) H(4.2) S(2.7) T(3.1) C(0.4)