Bioisis

"Folded Trimeric PF1787"

Experimental SAS Curve

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Experimental Mass

80,355 Da

Experimental Details for BID:  1ACSHP
Experiment ID: 5
Collected at: ALS BL 12.3.1
Contributors: Rambo, R.P. ,  Hura, G.L. ,  Menon, A.L. ,  Hammel, M. ,  Adams, M.W.W. ,  Tainer, J.A.
Part of a set of samples prepared recombinanlty by Mike Adams group in Georgia. Has His-tag attached, see sequence. The trimeric structure is surprising as a homolog had been crystallized in a dimeric form. Data is not on an absolute scale and is a compilation of several data sets collected at different energies.

Electron Pair Distribution

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       Dmax → 100 Å


Guinier Plot

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     Guinier Rg → 33.9 Å

Real Space Rg → 31.5 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 7.0.


DAMMIN/F Model

DAMMIN/F result was determined with the following:

Space GroupP1
NSD | RMSD0.703 
variance(NSD | RMSD)0.1 
Number of Models in Average10 

GASBOR Model

GASBOR result was determined with the following:

Space GroupP3
Chi1.72
NSD | RMSD1.2 
variance(NSD | RMSD)0.1 
Number of Models in Average10 
Superimposed model10 

Additional Experimental Details
Title

Folded Trimeric PF1787

Description

Part of a set of samples prepared recombinanlty by Mike Adams group in Georgia. Has His-tag attached, see sequence. The trimeric structure is surprising as a homolog had been crystallized in a dimeric form. Data is not on an absolute scale and is a compilation of several data sets collected at different energies.

Publication

Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS). Nat Methods. 2009 Aug;6(8):606-12.

Contributors

Rambo, R.P. ,  Hura, G.L. ,  Menon, A.L. ,  Hammel, M. ,  Adams, M.W.W. ,  Tainer, J.A.

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: ActylCoASY

Annotation: acetyl-CoA synthetase

AHHHHHHGSD RVAKAREIIE KAKAENRPLV EPEAKEILKL YGIPVPEFKV ARNEEEAVKF SGEIGYPVVM KIVSPQIIHK SDAGGVKINI KNDEEAREAF RTIMQNARNY KPDADLWGVI IYRMLPLGRE VIVGMIRDPQ FGPAVMFGLG GIFVEILKDV SFRVAPITKE DALEMIREIK AYPILAGARG EKPVNIEALA DIIVKVGELA LELPEIKEID INPIFAYEDS AIAVDARMIL
categoryamino acid composition(%)
HydrophobicI(12.5) V(8.3) L(6.2) M(2.9) A(10.8) G(6.7) P(6.2)
AromaticF(3.3) W(0.4) Y(2.5)
HydrophilicR(5.8) K(7.5) E(10.8) D(5.0) Q(1.2) N(3.3) H(2.9) S(2.5) T(0.8) C(0.0)