Bioisis

"28bp DNA duplex "

Experimental SAS Curve

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Experimental Mass

17,000 Da

Experimental Details for BID:  28BPDD
Experiment ID: 58
Collected at: ALS BL12.3.1
Contributors:
28bp DNA duplex standart SAXS profile for DNA.
Extrapolated to zero concentration from 4 different concentrations between 1 and 10 mg/mL

Electron Pair Distribution

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       Dmax → 100 Å


Guinier Plot

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     Guinier Rg → 26.6 Å

Real Space Rg → 27.0 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 1.7.


Additional Experimental Details
Title

28bp DNA duplex

Description

28bp DNA duplex standart SAXS profile for DNA.

Publication

Contributors

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: oligo

Annotation: e.g.,. C-terminal. ;ATP. binding. domain

5-GTGCTGAT GCGTCGTCGG ACTGATTCGG -3
categoryamino acid composition(%)
HydrophobicI(0.0) V(0.0) L(0.0) M(0.0) A(9.4) G(34.4) P(0.0)
AromaticF(0.0) W(0.0) Y(0.0)
HydrophilicR(0.0) K(0.0) E(0.0) D(0.0) Q(0.0) N(0.0) H(0.0) S(0.0) T(25.0) C(18.8)