Bioisis

"Immunoglobulin-like domains 1 and 2 of the protein tyrosine phosphatase LAR3"

Experimental SAS Curve

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Experimental Mass

19,900 Da

Experimental Details for BID:  LAR12P
Experiment ID: 59
Collected at: ALS BL12.3.1
Contributors:
Immunoglobulin-like domains 1 and 2 of the protein tyrosine phosphatase LAR3
Extrapolated to zero concentration from 3 concentration

Electron Pair Distribution

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       Dmax → 62 Å


Guinier Plot

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     Guinier Rg → 20.5 Å

Real Space Rg → 20.1 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 1.2.


Additional Experimental Details
Title

Immunoglobulin-like domains 1 and 2 of the protein tyrosine phosphatase LAR3

Description

Immunoglobulin-like domains 1 and 2 of the protein tyrosine phosphatase LAR3

Publication

The immunoglobulin-like domains 1 and 2 of the protein tyrosine phosphatase LAR adopt an unusual horseshoe-like conformation, Journal of molecular biology (2011) 408, 616-627

Contributors

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: MLAR1-2

Annotation: The. Immunoglobulin-like. Domains. 1. and. 2. of. the. Protein. Tyrosine. Phosphatase. LAR

GPGSSRGDSK PVFVKVPEDQ TGLSGGVASF VCQATGEPKP RITWMKKGKK VSSQRFEVIE FDDGAGSVLR IQPLRVQRDE AIYECTATNS LGEINTSAKL SVLEEDQLPS GFPTIDMGPQ LKVVEKGRTA TMLCAAGGNP DPEISWFKDF LPVDPAASNG RIKQLRSGAL QIESSEESDQ GKYECVATNS AGTRYSAPAN LYVRRVRRVA PRFS
categoryamino acid composition(%)
HydrophobicI(4.2) V(7.9) L(6.1) M(1.4) A(7.9) G(9.3) P(7.0)
AromaticF(3.7) W(0.9) Y(1.9)
HydrophilicR(7.0) K(6.1) E(7.0) D(5.1) Q(4.7) N(2.8) H(0.0) S(9.8) T(5.1) C(1.9)