Bioisis

"Monomeric PF1674"

Experimental SAS Curve

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Experimental Mass

19,696 Da

Experimental Details for BID:  1TSPHP
Experiment ID: 6
Collected at: ALS BL 12.3.1
Contributors: Rambo, R.P. ,  Hura, G.L. ,  Menon, A.L. ,  Hammel, M. ,  Adams, M.W.W. ,  Tainer, J.A.
Part of a set of samples prepared recombinantly by Mike Adams group in Georgia. A sequence homolog of this protein has been crystallized and its calculated scattering profiles resemble that of this protein. See structural model. Has His-tag attached, see sequence. Data is not on an absolute scale and is a compilation of several data sets collected at different energies.

Electron Pair Distribution

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       Dmax → 58 Å


Guinier Plot

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     Guinier Rg → 16.7 Å

Real Space Rg → 16.88 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 1.0.


DAMMIN/F Model

DAMMIN/F result was determined with the following:

Space GroupP1
NSD | RMSD0.49 
variance(NSD | RMSD)0.1 
Number of Models in Average10 

GASBOR Model

GASBOR result was determined with the following:

Space GroupP1
Chi8.6
NSD | RMSD1.51 
variance(NSD | RMSD)0.1 
Number of Models in Average10 
Superimposed model10 

Additional Experimental Details
Title

Monomeric PF1674

Description

Part of a set of samples prepared recombinantly by Mike Adams group in Georgia. A sequence homolog of this protein has been crystallized and its calculated scattering profiles resemble that of this protein. See structural model. Has His-tag attached, see sequence. Data is not on an absolute scale and is a compilation of several data sets collected at different energies.

Publication

Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS). Nat Methods. 2009 Aug;6(8):606-12.

Contributors

Rambo, R.P. ,  Hura, G.L. ,  Menon, A.L. ,  Hammel, M. ,  Adams, M.W.W. ,  Tainer, J.A.

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: TYRSRPOSPH

Annotation: Tyrosine/serine phosphatase

AHHHHHHGSL KNVFVKKLSG DIMEVRFVDD YVAFSRMPYE DEIGELVKEF DAFVVLVEEF ELEYDLEKVK REVEVLHVPI PDFTAPSIEE LKYIVKWIDE KVKEGKKVLV HCYGGSGRSG TVVVAWLMYK YKLPLKEALL EVRTLKPSAV ETRDQLEVLK EFEKLLGTIV
categoryamino acid composition(%)
HydrophobicI(4.1) V(14.1) L(11.2) M(1.8) A(4.1) G(5.3) P(3.5)
AromaticF(4.7) W(1.2) Y(4.1)
HydrophilicR(3.5) K(10.6) E(12.9) D(5.3) Q(0.6) N(0.6) H(4.7) S(4.1) T(2.9) C(0.6)