Bioisis

"Glucose Isomerase qmax 0.55"

Experimental SAS Curve

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Experimental Mass

174,000 Da

Experimental Details for BID:  GISRUP
Experiment ID: 66
Collected at: ALS BL12.3.1
Contributors: Hammel, ML
Glucose isomerase - Standard protein with the SAXS collected qmax ~0.55Å
Extrapolated to zero concentration from 4 different concentrations between 1 and 10 mg/mL

Electron Pair Distribution

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       Dmax → 97 Å


Guinier Plot

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     Guinier Rg → 31.3 Å

Real Space Rg → 32.1 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 4.7.


Additional Experimental Details
Title

Glucose Isomerase qmax 0.55

Description

Glucose isomerase - Standard protein with the SAXS collected qmax ~0.55Å

Publication

Hammel et al

Contributors

Hammel, ML

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: GISRUB

Annotation: Xylose. isomerase. from. Streptomyces. rubiginosus.

NYQPTPEDRF TFGLWTVGWQ GRDPFGDATR RALDPVESVR RLAELGAHGV TFHDDDLIPF GSSDSEREEH VKRFRQALDD TGMKVPMATT NLFTHPVFKD GGFTANDRDV RRYALRKTIR NIDLAVELGA ETYVAWGGRE GAESGGAKDV RDALDRMKEA FDLLGEYVTS QGYDIRFAIE PKPNEPRGDI LLPTVGHALA FIERLERPEL YGVNPEVGHE QMAGLNFPHG IAQALWAGKL FHIDLNGQNG IKYDQDLRFG AGDLRAAFWL VDLLESAGYS GPRHFDFKPP RTEDFDGVWA SAAGCMRNYL ILKERAAAFR ADPEVQEALR ASRLDELARP TAADGLQALL DDRSAFEEFD VDAAAARGMA FERLDQLAMD HLLGAR
categoryamino acid composition(%)
HydrophobicI(2.8) V(4.9) L(10.1) M(1.8) A(12.2) G(9.3) P(4.9)
AromaticF(6.0) W(1.6) Y(2.3)
HydrophilicR(9.1) K(2.8) E(7.3) D(9.6) Q(2.8) N(2.6) H(2.6) S(2.8) T(4.1) C(0.3)