Bioisis

"DNA double-strand break repair protein MRE11 +ATP"

Experimental SAS Curve

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Experimental Mass

138,000 Da

Experimental Details for BID:  MRERAP
Experiment ID: 67
Collected at: ALS BL12.3.1
Contributors:
DNA double-strand break repair protein MRE11 +ATP
SAXS data collected and analyzed at the Advanced Light Source SIBYLS beamline (BL12.3.1) as described. Briefly, data were collected at a wavelength of 1.0 Å and sample-to-detector distance of 1.5 m. Purified M2R2-head protein at 6 mg mL

Electron Pair Distribution

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       Dmax → 130 Å


Guinier Plot

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     Guinier Rg → 41.6 Å

Real Space Rg → 40.7 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 1.2.


Additional Experimental Details
Title

DNA double-strand break repair protein MRE11 +ATP

Description

DNA double-strand break repair protein MRE11 +ATP

Publication

ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair. Nat Struct Mol Biol. 2011 , 18(4):423-31.

Contributors

Genomics and Proteomics

The experiment is composed of 2 genes/ORFs

Abbreviated name: Mre11

Annotation: Mre11. N-terminus. -2-365

GSHMMFVHIA DNHLGYRQYN LDDREKDIYD SFKLCIKKIL EIKPDVVLHS GDLFNDLRPP VKALRIAMQA FKKLHENNIK VYIVAGNHEM PRRLGEESPL ALLKDYVKIL DGKDVINVNG EEIFICGTYY HKKSKREEML DKLKNFESEA KNYKKKILML HQGINPYIPL DYELEHFDLP KFSYYALGHI HKRILERFND GILAYSGSTE IIYRNEYEDY KKEGKGFYLV DFSGNDLDIS DIEKIDIECR EFVEVNIKDK KSFNEAVNKI ERCKNKPVVF GKIKREFKPW FDTLKDKILI NKAIIVDDEF IDMPDNVDIE SLNIKELLVD YANRQGIDGD LVLSLYKALL NNENWKELLD EYYNTKFRG
categoryamino acid composition(%)
HydrophobicI(9.5) V(5.1) L(10.3) M(1.9) A(3.5) G(5.1) P(3.0)
AromaticF(4.9) W(0.5) Y(5.7)
HydrophilicR(4.1) K(11.7) E(8.7) D(8.9) Q(1.1) N(7.0) H(3.0) S(3.8) T(1.1) C(1.1)

Abbreviated name: Rad50

Annotation: Rad50. ATP-bindingregion

MSMILKEIRM NNFKSHVNSR IKFEKGIVAI IGENGSGKSS IFEAVFFALF GAGSNFNYDT IITKGKKSVY VELDFEVNGN NYKIIREYDS GRGGAKLYKN GKPYATTISA VNKAVNEILG VDRNMFLNSI YIKQGEIAKF LSLKPSEKLE TVAKLLGIDE FEKCYQKMGE IVKEYEKRLE RIEGELNYKR LKEMSNLEKE KEKLTKFVEY LDKVRRIFGR NGFQAYLREK YVPLIQKYLN EAFSEFDLPY SFVELTKDFE VRVHAPNGVL TIDNLSGGEQ IAVALSLRLA IANALIGNRV ECIILDEPTV YLDENRRAKL AEIFRKVKSI PQMIIITHHR ELEDVADVII NVKKDGNVSK VKING
categoryamino acid composition(%)
HydrophobicI(9.6) V(7.4) L(8.8) M(1.9) A(5.8) G(6.8) P(1.9)
AromaticF(5.2) W(0.0) Y(4.4)
HydrophilicR(5.2) K(10.7) E(9.9) D(3.8) Q(1.6) N(7.1) H(1.1) S(5.5) T(2.7) C(0.5)