Bioisis

"Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain"

Experimental SAS Curve

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Experimental Mass

76,000 Da

Experimental Details for BID:  ATG7CP
Experiment ID: 68
Collected at: ALS BL12.3.1
Contributors:
Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain
Extrapolated to zero concentration from 4 different concentrations between 1 and 10 mg/mL

Electron Pair Distribution

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       Dmax → 115 Å


Guinier Plot

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     Guinier Rg → 30.7 Å

Real Space Rg → 32.3 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 2.2.


Additional Experimental Details
Title

Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain

Description

Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain

Publication

Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway. Mol Cell. (2011) 44(3):451-61.

Contributors

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: ATG7CTD

Annotation: C-terminal of ATG7

DPLKIADQSV DLNLKLMKWR ILPDLNLDII KNTKVLLLGA GTLGCYVSRA LIAWGVRKIT FVDNGTVSYS NPVRQALYNF EDCGKPKAEL AAASLKRIFP LMDATGVKLS IPMIGHKLVN EEAQHKDFDR LRALIKEHDI IFLLVDSRES RWLPSLLSNI ENKTVINAAL GFDSYLVMRH GNRDEQSSKQ LGCYFCHDVV APTDSLTDRT LDQMCTVTRP GVAMMASSLA VELMTSLLQT KYSGSETTVL GDIPHQIRGF LHNFSILKLE TPAYEHCPAC SPKVIEAFTD LGWEFVKKAL EHPLYLEEID GLSVIKQEVE RLGNDVFEWE DDESDEIA
categoryamino acid composition(%)
HydrophobicI(6.2) V(6.8) L(13.0) M(2.4) A(6.8) G(5.3) P(4.1)
AromaticF(3.6) W(1.5) Y(2.4)
HydrophilicR(4.4) K(6.2) E(6.8) D(7.4) Q(2.7) N(3.8) H(2.7) S(6.8) T(5.0) C(2.1)