Bioisis

"Tetrameric PF1281 - Superoxide Reductase"

Experimental SAS Curve

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Experimental Mass

57,296 Da

Experimental Details for BID:  1SPXGP
Experiment ID: 7
Collected at: ALS BL 12.3.1
Contributors: Rambo, R.P. ,  Hura, G.L. ,  Hammel, M. ,  Menon, A.L. ,  Tainer, J.A. ,  Adams, M.W.W.
Part of a set of samples prepared recombinantly by Mike Adams group in Georgia. The SAXS curve calculated from the crystallographic structure matches the measured profile well. Data is not on an absolute scale and is a compilation of several data sets collected at different energies.

Electron Pair Distribution

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       Dmax → 80 Å


Guinier Plot

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     Guinier Rg → 22.1 Å

Real Space Rg → 22.1 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 3.3.


DAMMIN/F Model

DAMMIN/F result was determined with the following:

Space GroupP1
NSD | RMSD0.501 
variance(NSD | RMSD)0.1 
Number of Models in Average10 

GASBOR Model

GASBOR result was determined with the following:

Space GroupP1
Chi7.48
NSD | RMSD0.84 
variance(NSD | RMSD)0.1 
Number of Models in Average10 
Superimposed model10 

Additional Experimental Details
Title

Tetrameric PF1281 - Superoxide Reductase

Description

Part of a set of samples prepared recombinantly by Mike Adams group in Georgia. The SAXS curve calculated from the crystallographic structure matches the measured profile well. Data is not on an absolute scale and is a compilation of several data sets collected at different energies.

Publication

Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS). Nat Methods. 2009 Aug;6(8):606-12.

Contributors

Rambo, R.P. ,  Hura, G.L. ,  Hammel, M. ,  Menon, A.L. ,  Tainer, J.A. ,  Adams, M.W.W.

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: SuprOxide

Annotation: superoxide reductase

MISETIRSGD WKGEKHVPVI EYEREGELVK VKVQVGKEIP HPNTTEHHIR YIELYFLPEG ENFVYQVGRV EFTAHGESVN GPNTSDVYTE PIAYFVLKTK KKGKLYALSY CNIHGLWENE VTLE
categoryamino acid composition(%)
HydrophobicI(6.5) V(10.5) L(6.5) M(0.8) A(2.4) G(8.1) P(4.8)
AromaticF(3.2) W(1.6) Y(6.5)
HydrophilicR(3.2) K(8.1) E(13.7) D(1.6) Q(1.6) N(4.8) H(4.8) S(4.0) T(6.5) C(0.8)