Bioisis

"Solution state of Glycosyl hydrolase + C-terminus"

Experimental SAS Curve

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Experimental Mass

43,000 Da

Experimental Details for BID:  AT5GHP
Experiment ID: 70
Collected at: ESRF ID-2
Contributors:
Solution state of Glycosyl hydrolase + C-terminus
Extrapolated to zero concentration from 4 different concentrations between 1 and 10 mg/mL

Electron Pair Distribution

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       Dmax → 74 Å


Guinier Plot

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     Guinier Rg → 21.2 Å

Real Space Rg → 21.3 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 1.3.


Additional Experimental Details
Title

Solution state of Glycosyl hydrolase + C-terminus

Description

Solution state of Glycosyl hydrolase + C-terminus

Publication

Structural Basis of Cellulosome Efficiency Explored by Small Angle X-ray Scattering , THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 280, NO. 46, pp. 38562–38568

Contributors

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: AT5

Annotation: Glycosyl hydrolase

MYDASLIPNL QIPQKNIPNN DGMNFVKGLR LGWNLGNTFD AFNGTNITNE LDYETSWSGI KTTKQMIDAI KQKGFNTVRI PVSWHPHVSG SDYKISDVWM NRVQEVVNYC IDNKMYVILN THHDVDKVKG YFPSSQYMAS SKKYITSVWA QIAARFANYD EHLIFEGMNE PRLVGHANEW WPELTNSDVV DSINCINQLN QDFVNTVRAT GGKNASRYLM CPGYVASPDG ATNDYFRMPN DISGNNNKII VSVHAYCPWN FAGLAMADGG TNAWNINDSK DQSEVTWFMD NIYNKYTSRG IPVIIGECGA VDKNNLKTRV EYMSYYVAQA KARGILCILW DNNNFSGTGE LFGFFDRRSC QFKFPEIIDG MVKYAFGLIN
categoryamino acid composition(%)
HydrophobicI(7.6) V(6.8) L(4.7) M(3.4) A(6.1) G(7.4) P(3.7)
AromaticF(4.7) W(2.9) Y(5.0)
HydrophilicR(3.4) K(5.5) E(3.4) D(6.6) Q(3.2) N(10.8) H(1.8) S(6.3) T(4.7) C(1.8)