Bioisis

"Characterization of Cu,Zn Superoxide Dismutase from the pathogen Brucella abortus"

Experimental SAS Curve

Your browser doesn't support canvas.

Low_res_thumbnail

Experimental Mass

16,070 Da

Experimental Details for BID:  BASODP
Experiment ID: 77
Collected at: ALS BL 12.3.1
Contributors: Pratt, AJ ,  Didonato, M ,  Shin, DS ,  Kassmann, CJ ,  Bruns, CK ,  Tainer, JA ,  Getzoff, ED
Brucella abortus is a pathogen that causes brucellosis, presenting severe health and alimentary consequences impacting humans and animals worldwide. The pathogen contains periplasmic Cu,Zn superoxide dismutase, which detoxifies superoxide radicals generated by the host immune response. Here we describe the monomeric SAXS structure of B. abortus SOD (BaSOD). Structural characterization of the enzyme may enable development of more potent vaccines to globally combat the pathogenic organism.
Data were collected at 0.85 and 1.7 mg/mL, and profiles superimposed well. Gel filtration matched buffer was used for buffer subtraction. X-ray exposure data from 0.5 to 5 seconds for the 1.7 mg/mL sample were used for merging and further analyses.

Electron Pair Distribution

Your browser doesn't support canvas.

       Dmax → 43 Å


Guinier Plot

Your browser doesn't support canvas.

     Guinier Rg → 14.6 Å

Real Space Rg → 14.51 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

Your browser doesn't support canvas.

The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

Your browser doesn't support canvas.

Low_res_thumbnail

The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 0.7.


Additional Experimental Details
Title

Characterization of Cu,Zn Superoxide Dismutase from the pathogen Brucella abortus

Description

Brucella abortus is a pathogen that causes brucellosis, presenting severe health and alimentary consequences impacting humans and animals worldwide. The pathogen contains periplasmic Cu,Zn superoxide dismutase, which detoxifies superoxide radicals generated by the host immune response. Here we describe the monomeric SAXS structure of B. abortus SOD (BaSOD). Structural characterization of the enzyme may enable development of more potent vaccines to globally combat the pathogenic organism.

Publication

to be published

Contributors

Pratt, AJ ,  Didonato, M ,  Shin, DS ,  Kassmann, CJ ,  Bruns, CK ,  Tainer, JA ,  Getzoff, ED

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: BaSOD

Annotation: metalloenzyme; monomer

ESTTVKMYEA LPTGPGKEVG TVVISEAPGG LHFKVNMEKL TPGYHGFHVH ENPSCAPGEK DGKIVPALAA GGHYDPGNTH HHLGPEGDGH MGDLPRLSAN ADGKVSETVV APHLKKLAEI KQRSLMVHVG GDNYSDKPEP LGGGGARFAC GVIE
categoryamino acid composition(%)
HydrophobicI(2.6) V(8.4) L(7.1) M(2.6) A(7.8) G(15.6) P(8.4)
AromaticF(1.9) W(0.0) Y(2.6)
HydrophilicR(1.9) K(7.1) E(7.8) D(4.5) Q(0.6) N(3.2) H(7.1) S(4.5) T(4.5) C(1.3)