Bioisis

"Noise assessment at high q-values"

Experimental SAS Curve

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Experimental Mass

173,000 Da

Experimental Details for BID:  1GINTP
Experiment ID: 79
Collected at: ALS BL 12.3.1
Contributors: Rambo, RP
10 independent, 5 sec exposures of dilute glucose isomerase at 0.8 mg/ml. Two curves were deposited: 1) average I(q) and 2) median I(q). For further information, see Figure 10 in Supporting Information.
Size exclusion purified samples were injected at ~10 mg/mL. Purified samples eluted at ~0.8 mg/mL and were used directly for SAXS.

Electron Pair Distribution

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       Dmax → 96 Å


Guinier Plot

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     Guinier Rg → 33.36 Å

Real Space Rg → 33.05 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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No model fit was provided, this dataset was used to demonstrate propagation of experimental uncertainties from 10 independent measures at a fixed glucose isomerase concentration. The average is the average of 10 separate measurements with associated matching buffer and the median is the median for each I(q) measurement.

The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


No Model

No structural model was used to interpret the SAXS data, please read the following:

10 independent, 5 sec exposures of dilute glucose isomerase at 0.8 mg/ml. Two curves were deposited: 1) average I(q) and 2) median I(q). For further information, see Figure 10 in Supporting Information.


Additional Experimental Details
Title

Noise assessment at high q-values

Description

10 independent, 5 sec exposures of dilute glucose isomerase at 0.8 mg/ml. Two curves were deposited: 1) average I(q) and 2) median I(q). For further information, see Figure 10 in Supporting Information.

Publication

“Accurate assessment of mass, models and resolution by small-angle scattering” Nature 496, 477-481 (25 April 2013)

Contributors

Rambo, RP

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: 10AD

Annotation: Monomer. sequence

MNYQPTPEDR FTFGLWTVGW EGRDPFGDAT RRALDPVESV RRLAELGAHG VTFHDDDLIP FGSSDSEREE HVKRFRQALD DTGMKVPMAT TNLFTHPVFK DGGFTANDRD VRRYALRKTI RNIDLAVELG AETYVAWGGR EGAESGGAKD VRDALDRMKE AFDLLGEYVT SQGYDIRFAI EPKPNEPRGD ILLPTVGHAL AFIERLERPE LYGVNPEVGH EQMAGLNFPH GIAQALWAGK LFHIDLNGQN GIKYDQDLRF GAGDLRAAFW LVDLLESAGY SGPRHFDFKP PRTEDFDGVW ASAAGCMRNY LILKERAAAF RADPEVQEAL RASRLDELAR PTAADGLQAL LDDRSAFEEF DVDAAAARGM AFERLDQLAM DHLLGARG
categoryamino acid composition(%)
HydrophobicI(2.8) V(4.9) L(10.1) M(2.1) A(12.1) G(9.5) P(4.9)
AromaticF(5.9) W(1.5) Y(2.3)
HydrophilicR(9.0) K(2.8) E(7.5) D(9.5) Q(2.6) N(2.6) H(2.6) S(2.8) T(4.1) C(0.3)