Bioisis

"Purified Yeast tRNA-Phe"

Experimental SAS Curve

Your browser doesn't support canvas.

Low_res_thumbnail

Experimental Mass

23,798 Da

Experimental Details for BID:  1TPHER
Experiment ID: 9    In addition, this experiment links to experiment(s):  16
Collected at: ALS BL 12.3.1
Contributors: Rambo, R.P. ,  Hammond, J.A. ,  Filbin, M.E. ,  Kieft, J.S.
Native yeast tRNA-Phe purified through a micro size-exclusion column prior to SAXS data collection. The commercially obtained tRNA is a mixture, as judged by the chromatogram, however, there was a single major peak which was used for the analysis.

Electron Pair Distribution

Your browser doesn't support canvas.

       Dmax → 98 Å


Guinier Plot

Your browser doesn't support canvas.

     Guinier Rg → 24.3 Å

Real Space Rg → 25.6 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

Your browser doesn't support canvas.

The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


GASBOR Model

GASBOR result was determined with the following:

Space GroupP1
Chi1.519
NSD | RMSD0.84 
variance(NSD | RMSD)0.1 
Number of Models in Average
Superimposed model

Additional Experimental Details
Title

Purified Yeast tRNA-Phe

Description

Native yeast tRNA-Phe purified through a micro size-exclusion column prior to SAXS data collection. The commercially obtained tRNA is a mixture, as judged by the chromatogram, however, there was a single major peak which was used for the analysis.

Publication

Comparison and functional implications of the 3D architecture of viral tRNA-like structures. RNA, 15:294-307 (2007)

Contributors

Rambo, R.P. ,  Hammond, J.A. ,  Filbin, M.E. ,  Kieft, J.S.

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: tRNA-Phe

Annotation: Yeast tRNA-Phe. This reference sequence was provided by the Saccharomyces Genome Database.

GCGGACUUAG CUCAGUUGGG AGAGCGCCAG ACUGAAGAUC UGGAGGUCCU GUGUUCGAUC CACAGAGUUC GCA
categoryResidue composition (%)
Nucleotides G (32.9) C (23.3) A (21.9) U (21.9) T (0.0)
Mass: 23,798 Da | Total residues: 73