Bioisis

"BTB-CUL3 ubiquitin ligase"

Experimental SAS Curve

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Low_res_thumbnail

Experimental Mass

74,600 Da

Experimental Details for BID:  SFLflP
Experiment ID: 96
Collected at: ALS BL 12.3.1
Contributors: Weinkam, P ,  Hammel, M ,  Tainer, J ,  Webb, B ,  Sali, A ,  Schneidman-duhovny, D
BTB-CUL3 ubiquitin ligase
single concentration

Electron Pair Distribution

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       Dmax → 125 Å


Guinier Plot

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     Guinier Rg → 37.81 Å

Real Space Rg → 38.54 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


Ensemble Model

A ENSEMBLE model was determined using the following:

Simulation MethodAllosMod-FoXS 
Simulation AlgorithmMD 
Ensemble Size10000 
Selection MethodMES 
Member Size
Scoring Functionchi 
Score0.0 

Ensemble Fit

Med_res_ensemble_fit

The red line is the calculated SAXS profile from the ENSEMBLE model scaled to the experimental SAXS curve (cyan).


Additional Experimental Details
Title

BTB-CUL3 ubiquitin ligase

Description

BTB-CUL3 ubiquitin ligase

Publication

Contributors

Weinkam, P ,  Hammel, M ,  Tainer, J ,  Webb, B ,  Sali, A ,  Schneidman-duhovny, D

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: BTB-CUL3

Annotation: Part of ubiquitin family Dimer.

GSKVVKFSYM WTINNFSFCR EEMGEVIKSS TFSSGANDKL KWCLRVNPKG LDEESKDYLS LYLLLVSCPK SEVRAKFKFS ILNAKGEETK AMESQRAYRF VQGKDWGFKK FIRRDFLLDE ANGLLPDDKL TLFCEVSVVQ DSVNISGQNT MNMVKVPECR LADELGGLWE NSRFTDCCLC VAGQEFQAHK AILAARSPVF SAMFEHEMEE SKKNRVEIND VEPEVFKEMM CFIYTGKAPN LDKMADDLLA AADKYALERL KVMCEDALCS NLSVENAAEI LILADLHSAD QLKTQAVDFI NYHASDVLET SG
categoryamino acid composition(%)
HydrophobicI(3.5) V(7.1) L(10.3) M(3.5) A(8.3) G(4.5) P(2.2)
AromaticF(5.8) W(1.3) Y(2.2)
HydrophilicR(3.8) K(8.3) E(9.0) D(6.7) Q(2.6) N(5.1) H(1.3) S(8.0) T(2.9) C(3.5)